LGBTQ+ representation
The Owl House has been praised for featuring several LGBTQ+ characters, particularly the romance between Luz Noceda and Amity Blight. Series creator Dana Terrace first implied a romance between the two on July 7, 2020, when responding to a fan who posted a screenshot from the upcoming episode "Enchanting Grom Fright" on Twitter, which showed Amity putting her hands on Luz's shoulders and looking into her eyes. Claiming "there is no heterosexual explanation" for Amity's action, Terrace responded, "there really isn't". On August 8, 2020, the episode, written by Lee Knox Ostertag, aired, and it featured a scene in which Luz and Amity dance together while casting spells to defeat "Grom", a demon that manifests as their deepest fears. The animation supervisor for the show, Spencer Wan, referred to their intimate dance as "the gay thing" and the first time he got to "do anything even remotely queer".
On September 2, 2020, during a Reddit AMA, Dana Terrace confirmed that Amity is intended to be a lesbian and that Luz is bisexual. The two girls represent Disney's second animated LGBTQ+ characters after Sheriff Blubs and Deputy Durland in Gravity Falls, and the first to be unambiguously portrayed as such. In the episode "Understanding Willow", one of the main characters, Willow Park, is shown to have two dads. Some noted that the beginning of the show's second season, which began airing in 2021, continued to build out the relationship between Amity and Luz, with Luz reciprocating Amity's feelings at the end of "Escaping Expulsion" and both blushing at each other. Others praised Amity's character evolving outside her "relationship with Luz".
The series was nominated for a GLAAD Media Award for Outstanding Kids and Family Programming in 2021 and won a Peabody Award for "...giving queer kids a welcome template...to explore their own budding creative energies."
The episode "Through the Looking Glass Ruins" received significant attention over Luz and Amity's growing relationship and its ending, in which Amity kisses Luz on the cheek. The episode "Eda's Requiem" introduces a character named Raine Whispers, who is referred to with singular they pronouns. Raine is voiced by transgender and non-binary actor Avi Roque. Raine is Disney TVA's first transgender and/or non-binary character. Roque said that the character is based on their own experience, with the character's skin color reflecting their actual skin color, and praised the show for normalizing queer identity, and said it was an honor to voice Raine. In the episode, Eda Clawthorne is shown to have romantic feelings for Raine. The subsequent episode, "Knock, Knock, Knockin' on Hooty's Door", reveals that Eda and Raine were formerly dating, before breaking up due to Raine beginning work in the coven system. The episode also includes a scene of Luz and Amity asking each other out and officially becoming a couple. GLAAD praised the episode, saying they were excited to see a "wonderful and affirming message" from the series. Jade King of TheGamer praised the series for having a fictional universe where queer characters can "learn to love themselves without the fear of ridicule", comparing it to the similar approaches in Steven Universe and She-Ra and the Princesses of Power, noting the relationship between Luz and Amity.
In March 2022, Lilith, Eda's older sister, was revealed to be aromantic and asexual during a charity Livestream, via an in-character letter read by the character's voice actress Cissy Jones. Jade King of TheGamer noted that Cissy Jones said that her letter during a charity stream saying that Lilith didn't have any romantic attractions was "basically canon".
On May 21, 2022, the penultimate episode of season two, "Clouds on the Horizon" aired, in which Luz and Amity share a kiss on the lips.
A promotional video released on September 25, 2022, depicted Luz in a new outfit with a pin on her beanie, picturing the bisexual flag. On October 15, 2022, the first The Owl House special "Thanks to Them" has Luz coming out to her mother as bisexual. The special also reveals that Vee's campmate Masha is non-binary: at the character's workplace, their desk has a name plate that reads "MASHA (they/them)", and their nails are painted the colors of the non-binary pride flag. On April 8, 2023, the series finale "Watching and Dreaming" revealed King's father "Papa Titan" to be genderqueer, as he refers to himself as "both king and queen".
After the series' finale, Dana Terrace clarified two of Luz's friends, Willow and Hunter, as being pansexual and bisexual respectively.
