Family connections
Anne's family background was a dense web of relationships involving the Mercers' Company, the court, Marian exiles and notable religious figures. Her father, Stephen Vaughan, was a merchant and diplomatic agent for Henry VIII. Her mother Margaret or Margery was firstly the wife of Edward Awpart, citizen and Girdler of London in the parish of St Mary le Bow, who originated from Penkridge in Staffordshire. The Awparts had five children, Elizabeth, Anne, Joan, Edward and Susan, who were all unmarried at the time of their father's death in 1532. Through his connection to Thomas Cromwell, Stephen Vaughan found a position for Anne's mother as silkwoman to Anne Boleyn. His second wife, Anne's stepmother Margery, was the widow of Henry Brinklow, mercer and polemicist, and went on to make two further marriages.
Henry Lok was a mercer and one of many children of the mercer William Lok, who married four times; William Lok was also connected to Cromwell. Anne's sister-in-law, and one of Henry Lok's sisters, was Rose Lok (1526–1613), known as a Protestant autobiographical writer, married to Anthony Hickman. Another of Henry Lok's sisters, Elizabeth Lok, married Richard Hill; both Rose and Elizabeth were Marian exiles. Elizabeth later married Bishop Nicholas Bullingham after his first wife died (1566). Michael Lok was a backer of Martin Frobisher, and married Jane, daughter of Joan Wilkinson, an evangelical associate of Ann Boleyn and her chaplain William Latimer.